Table 2.
Bio-plex™ nine-cytokine measurement characteristics
| Analyte | Sensitivity and range (pg/ml) |
Positive controls (pg/ml) (% recovery) |
||||||
|---|---|---|---|---|---|---|---|---|
| Serum | Tissue | 100 |
800 |
4000 |
||||
| Serum | Tissue | Serum | Tissue | Serum | Tissue | |||
| IL-1α | 2–16 000 | 4–16 000 | 91 | 96 | 102 | 104 | 96 | 108 |
| IL-1β | 2–32 000 | 2–4000 | 96 | 97 | 100 | 110 | 98 | 99 |
| IL-2 | 2–16 000 | 2–16 000 | 99 | 85 | 103 | 170 | 97 | 85 |
| IL-4 | 2–32 000 | 2–4000 | 101 | 90 | 96 | 110 | 142 | 134 |
| IL-6 | 2–32 000 | 8–32 000 | 100 | 109 | 101 | 111 | 105 | 96 |
| IL-10 | 2–32 000 | 50–32 000 | 98 | 90 | 116 | 111 | 102 | 104 |
| TNF-α | 4–32 000 | 2–32 000 | 81 | 98 | 93 | 111 | 91 | 108 |
| IFN-γ | 2–4000 | 4–32 000 | 87 | 107 | 117 | 106 | 57 | 106 |
| GM-CSF | 2–8000 | 4–4000 | 101 | 91 | 100 | 111 | 95 | 91 |