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. Author manuscript; available in PMC: 2010 Jan 4.
Published in final edited form as: Neurobiol Aging. 2007 Jun 28;29(12):1923–1929. doi: 10.1016/j.neurobiolaging.2007.05.002

Figure 2.

Figure 2

Allele-specific MAPT expression of N-terminal exons in a neuronal cell culture model and human post-mortem brain tissue. The expression data is normalised to the equivalent of each SNP 1 allele being present in one copy on genomic DNA to produce a standardized transcript ratio. A ratio of > 1 at SNP 1 indicates more MAPT transcript isoform from H2 than H1. (A) Allele-specific expression of N-terminal MAPT exons in differentiated SK-N-F1 cells. Mean values and standard deviations from eight measurements using the reverse primer are shown. 0N H2:H1 MAPT transcript ratio = 0.88, S.D. = 0.02, p = 0.0002. 1N H2:H1 MAPT transcript ratio = 1.33, S.D. = 0.04, p < 0.0001. 2N H2:H1 MAPT transcript ratio = 2.30, S.D. = 0.28, p = 0.0007. (B) Allele-specific expression of N-terminal MAPT exons in each of the fourteen H1/H2 heterozygotes measured using SNP 1. Mean values and standard deviations from up to sixteen measurements (eight measurements using the forward primer and eight measurements using the reverse primer) are shown. (C) Mean values and standard deviations of relative H2:H1 expression averaged from all fourteen H1/H2 heterozygotes.