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Effect of rumen fluid collecting time (0, 3, 6, 9 and 12 h) after feeding on aflatoxin B1 (AFB1) degradation. Rumen fluid was supplemented with AFB1 to a final concentration of 100 ppb. *Significantly different between goat and steer (p < 0.01). Incubation of rumen fluid was done at 39℃ and collected at times of 0, 3, 6, 9, and 12 h after feeding the animal. AFB1 degradation assay was performed by ELISA.
