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Wild type (WT), nblRΩ, (RΩ), and nblAΩ (AΩ) were spotted onto nutrient-replete solid medium after various times (24, 48, and 72 h) of starvation for sulfur (−S) or nitrogen (−N) in liquid medium. Each spot consisted of 10 μl from a 2-fold dilution of the original culture. Where indicated, the cultures were made 5 μM of DCMU upon initiation of starvation (t0).
