Fig. 1.

Standard curves for the multiplex real-time PCR for Salmonella (S.) Typhimurium. The results of the multiplex real-time PCR were determined using decimal dilution of S. Typhimurium ATCC 14028 DNA. The PCR reaction contained primers and probes for all Salmonella spp., S. Typhimurium and S. Enteritidis. Vertical (y) axis, fluorescence intensity; horizontal (x) axis, PCR cycle numbers. Standard curves for the multiplex real-time PCR of S. Typhimurium. The reactions of S. Typhimurium were always positive at 555 nm (JOE) and 510 nm (FAM). The threshold values (C_T) were plotted against the corresponding bacterial cell number (log₁₀ CFU/ml).