Figure 2.

Effect of MAA on endogenous ERα expression in vitro and in vivo. (A) MCF-7 cells were treated for 24 hr with either vehicle [ethanol (EtOH)] or increasing concentrations of MAA, and ERα protein expression was assessed by Western blot. Data are representative of results from three independent experiments. (B) MCF-7 cells were treated for 24 hr with either vehicle or 5 mM MAA, and ERα mRNA levels were measured by real-time PCR. Data represent the average fold over control (± SE) obtained from duplicate samples in four independent experiments. (C) Uteri were collected from mice treated for 2.5 hr with either saline or 400 mg/kg MAA, and real-time PCR was performed to determine the levels of ERα mRNA in each sample. Data are plotted as fold over control (± SE) and represent the average values obtained from three mice per treatment.

^##p < 0.01 compared with vehicle control.