Figure 2.

Alignment of the A. thaliana (A.t.) MetRS amino acid sequence with the Synechocystis (Syn) and E. coli (E.c.) MetRS sequences. Optimal sequence alignment was obtained by using pileup (Univ. of Wisconsin Genetics Computer Group). Amino acids in the A. thaliana sequence that are conserved either in the Synechocystis or in the E. coli protein are highlighted. The class I aminoacyl–tRNA synthetase signatures HIGH and KMSKS are underlined with filled triangles. The C-terminal amino acid of the presequence that was fused to the GFP is indicated by an open arrow. The N-terminal amino acid of the protein expressed in E. coli is indicated by a filled arrow.