Transient expression of pCK-MetPS-GFP3 (A–D), RecA–GFP (E), and COXIV–GFP (F) in tobacco cells. A and B, and C and D, respectively, are two views of the same cell. GFP was observed by using a GFP(R)-BP set of filters (A and C), GFP plus chlorophyll fluorescence with a GFP(R)-LP set of filters (B, E, and F), and 4-chloromethyltetramethylrosamine staining of mitochondria with a tetramethylrhodamine B isothiocyanate filter set (D). Examples of plastids (p) and mitochondria (m) are pointed out. (×100; bars = 1 μm.)