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. 2009 Oct 22;285(2):1053–1065. doi: 10.1074/jbc.M109.044529

FIGURE 4.

FIGURE 4.

Surface plasmon resonance analyses of FH fragments with immobilized CRP. A and B, SCR-16/20 binding in 50 mm and 137 mm NaCl buffer. Here, 10, 20, and 50 μm SCR-16/20 bind to CRP, and 8 μm C3u was used as a nonbinding control. C and D, the Tyr-402 and His-402 allotypes of SCR-6/8 binding in 50 mm and 137 mm NaCl buffer. Here, 5, 10, 15, and 20 μm SCR-6/8 allotypes bind differentially to CRP, and 5 μm SCR-1/5 was used as a nonbinding control. E and F, equilibrium KD analyses for SCR-16/20 and the SCR-6/8 allotypes binding to immobilized CRP in 137 mm NaCl. The SCR-16/20 concentration ranged between 0 and 15 μm, and that for SCR-6/8 ranged between 0 and 20 μm. The KD values were determined using an affinity 1:1 model (insets in E and F).