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Binding of NmFNR to DNA measured using fluorescence anisotropy. The change in fluorescence anisotropy of 2 nm duplex 40-mer DNA containing a consensus FNR box (filled circles) or lacking an FNR consensus sequence (open circles) was measured on titrating increasing concentrations of anaerobic NmFNR. An increase in anisotropy was observed with the DNA containing the FNR consensus sequence and the data fitted to a hyperbolic curve. Error bars represent S.D. from the mean.
