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. 2009 Oct 30;285(2):1208–1219. doi: 10.1074/jbc.M109.035436

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FIGURE 2. — OPN and BSP are potential in vivo substrates of ppGalNAcT-1. Total bone extracts prepared from calvaria and tibia of C57BL/6 wild-type (+/+) and Galnt1-null (−/−) mice were separated on 4–12% NuPAGE gel and stained with (A) Stains-all, or transferred onto polyvinylidene difluoride membrane for immunoblot analysis against OPN (2A1) (B) and BSP (LF-84) (C). Protein species that migrated differently between the wild-type and Galnt1-null mice are marked with arrows. Bands marked with asterisks in B and C are multimers likely formed by transglutamination.