TABLE 3.

Kinetic parameters of NylA mutant enzymes

Acd hydrolytic activity was assayed using the purified enzymes under standard assay conditions, except that various concentrations of Acd were used.

Mutation	Kinetic study
	Km	kcat	kcat/Km
	mm	s−1	s−1mm−1
Wild type	3.51 ± 0.16	4.97 ± 0.10	1.42
N125A	13.2 ± 0.75	1.12 ± 0.04	0.085
C316A	0.85 ± 0.04	0.71 ± 0.01	0.83
C316S	1.80 ± 0.20	4.83 ± 0.17	2.68
C316G	3.66 ± 0.27	9.15 ± 0.28	2.50
C316D	19 ± 10	0.14 ± 0.05	0.007