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. 2009 Nov 10;285(2):1343–1357. doi: 10.1074/jbc.M109.024703

FIGURE 1.

FIGURE 1.

Kidins220/ARMS expression and distribution in hippocampal neurons in vitro. A, lysates from different developmental stages (DIV) of rat hippocampal neurons in culture or rat brain from embryonic (E), postnatal (P, days), or adult (M; months) animals were immunoblotted with anti-Kidins220/ARMS antibody. β-Actin was used as a control. B, hippocampal neurons were fixed at stage 1 (a), 2 (b), or 3 (c) and immunostained with phalloidin-TRITC (F-Actin, red). Scale bar, 10 μm. C and D, hippocampal neurons were fixed at stage 2 and immunostained with anti-Kidins220/ARMS (green) and α-tubulin (C; red) or the Golgi marker GM130 (D; red). Note that Kidins220/ARMS co-localizes with the Golgi apparatus in the juxtanuclear region. Nuclei stained with DAPI (blue) staining are shown in the merged image. Scale bar, 10 μm.