FIGURE 5.

Down-regulation of Kidins220/ARMS in hippocampal neurons alters normal dendrite development. A, hippocampal neurons were transfected with GFP-shC (a) as control or GFP-sh2 Kidins220/ARMS-specific shRNA (b). After 5 DIV, neurons were fixed and immunostained for the dendritic marker MAP2 (red). Similar to non-transfected neurons, GFP-shC neurons developed normal dendrites, whereas GFP-sh2 neurons displayed aberrant MAP2-positive processes. Compare the green neuron where Kidins220/ARMS has been knocked down with the neighbor neuron. Scale bar, 20 μm. B, quantification of the percentage of neurons at stage 4 bearing normal or aberrant dendritic processes. The data shown are the means ± S.E. of three independent experiments (shC n = 100 cells, sh2 n = 100 cells), and statistical significance was evaluated by Student's unpaired t test (**, p < 0.01).