FIGURE 10.

Stimulation of CB1R and IL-6R results in enhanced neurite outgrowth in primary rat cortical neurons. Rat primary cortical neuron cultures were treated with IL-6 and/or HU-210 at 2 DIV and fixed after 24 h. The cells were labeled for MAP2 (red) and Tau (green) to identify developing neurites. A, representative confocal images of cells under each condition are shown. B, the lengths of the longest neurite of each cell were measured morphometrically. The bar graph shows mean ± S.E. for average neurite length and median neurite length for each treatment. Statistical differences were calculated using the one-tailed Wilcoxon Mann-Whitney test for medians; *, p < 0.05; ***, p < 0.0005. See supplemental Table 2S for statistical significance comparisons of all conditions.