FIGURE 2.

Stimulation of CB1R and IL-6R results in enhanced early MAPK phosphorylation. N2A cells were serum-starved for 24 h and treated with 0.5 ng/ml IL-6 (A), 100 nm HU-210 (B), or both (C) for the indicated periods of time. The cells were lysed and subjected to immunoblot analysis for phospho-MAPK, total MAPK, and tubulin. The blots shown are representative of three independent experiments. D, quantification of MAPK phosphorylation represented as phospho-MAPK (p-MAPK)/total MAPK ratio over time.