FIGURE 7.
Activation of CB1R and IL-6R results in enhanced STAT3 phosphorylation at 3 h of treatment. N2A cells were serum-starved for 24 h and treated with 0.5 ng/ml IL-6 (low), 5 ng/ml IL-6 (hi), 100 nm HU-210 (low), 1 μm HU-210 (hi), and a combination of low doses for 3 h. The cells were lysed and subjected to immunoblot analysis for phospho-STAT3 (phospho-Tyr705), total STAT3, and tubulin. A, the blot shown is representative of three independent experiments. B, quantification of STAT3 phosphorylation represented as phospho-Tyr705/STAT3 ratio.