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Gel filtration chromatography profiles for mixtures of OsRbohB-(138–313) and OsRac1. Fractions were analyzed by SDS-PAGE. Peaks corresponding to input proteins and the complex are labeled. A, a complex fraction can be detected in a 1 mm:40 μm mixture of OsRbohB-(138–313) and OsRac1. B and C, no complex fraction is present in 40 μm:1 mm and 40 μm:40 μm mixtures. D, a complex fraction and a residual peak for OsRac1 are detectable in a 1:1 mm mixture.
