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. 2009 Nov 4;285(2):1479–1489. doi: 10.1074/jbc.M109.057141

FIGURE 2.

FIGURE 2.

Expression levels of hsa-miR-7 and mmu-miR-706 are decreased during intestinal epithelial cell differentiation. A, schematic representation of the human (h) CD98 mRNA with the 3′-UTR hsa-miR-7 binding site (upper panel) and sequence alignment of hCD98 3′-UTR miR-7 target site (bottom panel) predicted by miRBase. B, levels of mature forms of hsa-miR-7 or hsa-miR-626, used as a control miRNA, in Caco2-BBE cells cultured on plastic plates for 2, 5, and 8 days were analyzed by real-time RT-PCR. C, schematic representation of the mouse (m) CD98 mRNA with the 3′-UTR mmu-miR-706 binding site (upper panel) and sequence alignment of mCD98 3′-UTR mmu-miR-706 target site predicted by miRBase. D, levels of mature form of mmu-miR-706 in the villus and crypt fractions isolated from jejunum of 6–8-week-old FVB male mice were quantified by real-time RT-PCR. Values represent means ± S.E. of three determinations. *, p < 0.05; **, p < 0.005; NS, not statistically significant.