FIGURE 5.

MiR-7 antisense increases hCD98 expression in Caco2-BBE cells. A, Caco2-BBE cells were transfected with the hCD98 3′-UTR-luciferase reporter construct in the absence (control) or presence of 40 nm miR-7 antisense or miR-control antisense, or 40 nm miR-7 precursor. Luciferase activity was measured at 2 days post-transfection and normalized to lysate protein concentration. B, Caco2-BBE cells were transfected with vehicle (Lipofectamine 2000; control), 40 nm miR-7 antisense or miR-control antisense, or 40 nm miR-7 precursor for 2 days. hCD98 expression was assessed by Western blot. Bar graphs show the relative intensity of blots in the upper panel. *, p < 0.05; **, p < 0.005; ***, p < 0.001; NS, not statistically significant versus control.