FIGURE 3.

Steady-state DNA polymerization assays. pol γA with or without pol γB (wild-type or a variant) were analyzed using M13mp18 DNA annealed to a 26-nt primer. A, reaction products were visualized on a polyacrylamide denaturing gel. Reactions contained 80 nm pol γA, 200 nm pol γB or a variant, 50 nm primer-template DNA, and 10-fold excess of trap DNA. B, reactions were performed with pol γA either alone at 40, 80, and 160 nm, or in the presence of ΔI4-D129K at concentrations of pol γA/ΔI4-D129K 40 nm/100 nm, 80 nm/200 nm, or 160 nm/400 nm. pol γA/wild-type pol γB 40 nm/100 nm served as the control.