TABLE 1.
pol γB proteins | Kda | Molecular massb | Oligomeric state |
---|---|---|---|
μm | kDa | ||
Wild-type | <0.1 | 92.9 (89.5, 94.7) | Dimer |
D129K | <0.1 | 91.1 (90.8, 92.0) | Dimer |
ΔI4 | 16.6 | 90.9 (90.7, 91.3) | Monomer/dimer mixture |
78.1 (77.8, 79.1) | |||
ΔI4-D129K | >200 | 43.8 (43.6, 44.1) | Monomer |
ΔI4-D129K/R107E | NDc | Monomer/dimer mixture |
a Measured by analytical ultracentrifugation using the sedimentation equilibrium method.
b Based on genetic algorithm-Monte Carlo analysis of sedimentation velocity data. Values in parenthesis are 95% confidence intervals.
c ND, not determined.