FIGURE 2.

TAT PTD-Cre fusion protein cell association and cell-surface binding in glycan-deficient cells. A, flow cytometric analysis of cells treated for 1 h at 37 °C at the indicated concentrations of recombinant TAT PTD-Cre labeled with Alexa Fluor 546. Cells were washed thoroughly with PBS, heparin, and trypsin to remove extracellular TAT fusion protein. B, live cell photomicrographs of the indicated cell lines treated with 1 μm TAT PTD-Cre-Alexa Fluor 546 for 1 h at 37 °C. TAT PTD-Cre treatment was followed by washes with PBS and heparin. Scale bar = 5 μm. C, TAT PTD-Cre-Alexa Fluor 546 cell-surface binding at 4 °C. Cells were treated for 15 min with TAT PTD-Cre-Alexa Fluor 546, followed by PBS wash, nonenzymatic cell dissociation, and flow cytometry. WT, wild-type.