FIGURE 1.

Immunoelectron microscopy studies of FBPase distribution in wild-type and Δpep4 cells following the addition of glucose. A, wild-type cells were glucose-starved and transferred to media containing fresh glucose for 15 min. Cells were fixed and embedded. Immuno-EM was performed on thin sections of cells using affinity-purified FBPase antibodies followed by goat anti-rabbit antibodies conjugated with 10 nm colloid gold. B, Δpep4 cells were glucose-starved and transferred to media containing glucose for 15 min. Cells were fixed, and immuno-EM was performed on thin sections of cells. C, enlarged views of FBPase distribution in areas near the plasma membrane. CW, cell wall; PM, plasma membrane.