FIGURE 4.

FBPase degradation is defective in mutants that block early steps of endocytosis. A, wild-type and various mutant cells that block early steps of endocytosis were grown in low glucose media. Cells were transferred to media containing fresh glucose for 0, 2, and 3 h, and FBPase degradation was examined. B, wild-type and endocytosis mutants were transferred from low to high glucose for 15 min. Cell lysates were subjected to differential centrifugation and FBPase distribution in the cytosolic (C) and Vid vesicle (V)-enriched fractions was examined. FBPase levels in these fractions were quantitated using ImageJ software (National Institutes of Health).