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FBPase remains in the cytosol when early steps of endocytosis are blocked. The Δvph1 cell expressing FBPase-GFP (A), Δsla1 cells expressing FBPase-GFP (B), and Δrvs167 cells expressing FBPase-GFP (C) were grown in low glucose media. Cells were transferred to media containing fresh glucose in the presence of FM for the indicated times. FBPase-GFP, FM, and cells were visualized using fluorescence microscopy.
