FIGURE 6.

FBPase and MDH2 associate with actin patches following the addition of glucose. A, wild-type cells expressing Abp1p-GFP were grown in YPKG media for 3 days. The localization of Abp1p-GFP with rhodamine-conjugated phalloidin was examined. B, wild-type cells expressing FBPase-GFP were transferred to media containing fresh glucose for the indicated times. Cells were fixed and processed as described under “Experimental Procedures.” Actin was stained with phalloidin conjugated with rhodamine. FBPase, actin, and cells were visualized by fluorescence microscopy. C, MDH2-GFP was expressed in Δmyo3 cells that were transferred to media containing glucose for 0–60 min. MDH2 and actin localization was examined by fluorescence microscopy.