FIGURE 2.

PMA-induced translocation of PKC isoforms. HL-60 (A) or Jurkat cells (B) were treated for 1 h with the indicated concentration of PMA. After washing, mechanical disruption, and removal of nuclei by sedimentation at 800 × g, the postnuclear supernatant was fractionated into cytosol (280,000 × g supernatant) and membranes (280,000 × g pellet). Aliquots containing 50 μg of protein were subjected to SDS-PAGE, transferred to nitrocellulose, and probed with antibodies to the indicated antigen. Dashed line in B indicates juxtaposition of nonadjacent wells from the same piece of x-ray film.