FIGURE 2.

The effects of interface mutations of S100A4 on metastasis and invasion in vivo and S100A4-induced migration of Rama 37 cells in vitro. Cell pools or clones of Rama 37 cells expressing wild type S100A4 or mutants 72, 72.78, or 16.72.78 were subjected to migration assays in quadruplicate Transwell chambers as described under “Experimental Procedures.” Parental Rama 37 cell line and cells transfected with empty vector were used as negative controls. For statistical analysis, at least four independent assays were conducted on clones and four on pools of cells. The results were converted to a percentage of wild type migration. The horizontal line denotes the basal level of migration of the Rama 37 cells in the Transwell assay. Incidences of metastasis and invasion are expressed as the percentages of animals with primary tumors using data from Table 1.