FIGURE 4.

Elution profile of S100A4 wild type and mutant proteins on gel filtration chromatography. Samples were applied to a Superdex 200 10/300 GL column equilibrated with TBS in the absence of (left-hand column) or in the presence of 0.5 mm Ca²⁺ (right-hand column). Proteins were separated at a flow rate of 0.5 ml/min, and elution was monitored at 215 nm. The retention times were related to those of standard proteins (“Experimental Procedures”) to determine the molecular masses of the S100A4 wild type and mutant proteins.