FIGURE 5.

Identification of SAP97 alternative splice variants in rat brain at P21. A, primers (shown by arrows) were designed to amplify mRNAs encoding the SH3 domain and the entire U5 region of SAP97. B, left, analysis of the reverse transcription-PCR reaction using RNA from P21 rat brain as template; the expected 400–500-bp DNA band (arrowhead) is missing from controls lacking either reverse transcriptase (RTase) or template mRNA. Right, representative agarose gel showing individual clones of different sizes chosen for sequence analysis (asterisks). C, schematic illustrating four U5 splice variants detected among the total of 24 clones analyzed that contained distinct combinations of the I2, I3, I5, and/or full-length and short forms of the I4 insert (I4 and I4s, respectively). Amino acid sequences of I4 and I4s inserts are shown below.