Table I.
Nucleotide sequences of the primers used in this study (GenBank reference numbers: AF240778 for toxA gene in P. multocida and AY048744 for kanR gene in pKD13)
| Primers | Nucleotide sequence (5′– 3′) | |
|---|---|---|
| P1 | toxA-N-F | GCGC CTCGAG* ATGAAAACAAAACATTTTTT |
| P2 | toxA-N-R | GCGC AGATCT* GAGTAATGAAGAGCATAGT |
| P3 | toxA-C-F | GCGC GGTACC* ATTGACTTTTTCCTAAATAA |
| P4 | toxA-C-R | AATT GGATCC* TTATAGTGCTCTTGTTAAGC |
| P5 | kanR-F | GGCC AGATCT* ATGATTGAACAAGATGGAT |
| P6 | kanR-R | AATT GGTACC* TCAGAAGAACTCGTCAAGA |
*
Underlined: enzyme sites.