Table II.

Experimental design for toxigenicity test and protection study. Mice in groups 1 and 2 were vaccinated with live PMT mutant P. multocida bacterial culture, while controls (groups 3 and 4) with PBS. For challenge study, group 2 and 4 were challenged with W/T P. multocida, while group 1 and 3 with W/T bacterial lysate

Group	Route	Dosage per mouse	Trial number	Challenge with
1	Intraperitoneal	PMT mutant (1 × 107 cells)	12	Bacterial lysate 500 μL
2	Intraperitoneal	PMT mutant (1 × 107 cells)	12	Bacterial culture (1 × 107 cells)
3	Intraperitoneal	PBS 100 μL	12	Bacterial lysate 500 μL
4	Intraperitoneal	PBS 100 μL	12	Bacterial culture (1 × 107 cells)