Table I.
Comparative analyses of Streptococcus suis S735 and its unencapsulated mutant BD101 for lectin-binding, fibrinogen-binding, and chymotrypsin-like activities
| Activity |
S. suis |
% change (parent versus mutant) |
|
|---|---|---|---|
| S735 | BD101 | ||
| Lectin-binding activity (RFU) | |||
| WGA | 1859 ± 54 | 431 ± 46 | −77a |
| SBA | 743 ± 40 | 658 ± 45 | −11 |
| MPA | 613 ± 19 | 572 ± 23 | −7 |
| Fibrinogen-binding activity (ng bound/well) | 193 ± 10 | 136 ± 7 | −30a |
| Chymotrypsin-like activity (A405) | 1.25 ± 0.07 | 0.86 ± 0.04 | −31a |
RFU — Relative fluorescence units; WGA — wheat germ agglutinin from Triticum vulgaris, specific for N-acetyl glucosamine and sialic acid residues; SBA — soybean agglutinin from Glycine max, specific for N-acetyl galactosamine and galactose residues; MPA — Maclura pomifera agglutinin, specific for N-acetyl galactosamine residues.
a
Significant difference (P < 0.01) with the Student’s t-test between S. suis S735 and its unencapsulated mutant BD101.