Fig.3.
Triskelion dimerization and cage formation occurring in “assembly buffer” (0.1 M MES, 3mM CaCl2, 0.5 mM MgCl2, 1mM EDTA, pH 6.2.), imaged in air following drying on mica (see Methods). (A) Topographs (two height scales and the XY scale to the right) reveal individual triskelia, dimers and small oligomeric profiles (2 nm fire-color scale), and larger assemblies of varying sizes (20 nm mono-green scale, within masked patches). (B) Sections through individual triskelia show hub and terminal domains measuring between 1 nm and 2 nm in height. (C),(D) Histograms of triskelion arm contour length, L, and the distance between terminal domains, Dee, as defined in (B) (see, also, Supplemental Table 1).