Cysteine 111 is not required for the co-aggregation of WT hSOD1 with G85R hSOD1. A. Immunoblots of detergent extracted cells that co-expressed C111S and G85R hSOD1 proteins for 48h. UT: untransfected cells. hWT: cells transfected with WT hSOD1 construct. Mutant SOD1 constructs noted by G85R, and C111S notation. B. Quantification of the elative aggregation propensity of mutant hSOD1 proteins. Bars represent mean ± SEM. N=4. Statistical analysis compares the aggregation of WT hSOD1 to each mutant as indicated on the figure (A4V, G85R, or C111S), *p < 0.05, #p < 0.005. Comparison is also made between C111S alone to C111S in cells co-transfected with G85R, *p < 0.05.