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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Methods. 2009 May 4;49(3):270–274. doi: 10.1016/j.ymeth.2009.04.011

Figure 1.

Figure 1

A) Schematic of RNP interaction with target site. Example shows the target site within the F. tularensis blaB gene. LtrA has preferences for basepairs shown at −23, −21, −20, and +5 in relation to the insertion site (designated with Δ), while the EBS2 and EBS1-δ loops of the LtrB RNA basepair with the target sequences shown. The targetron algorithm identifies potential recognition sites within genes based on the LtrA preferences, while the SOE PCR with specific oligonucleotides alters EBS2 and EBS1 within LtrB to specifically basepair to the target sequence, resulting in specific insertion of the intron into the target gene. B). Map of the F. tularensis targetron plasmid pKEK1140, with the features outlined in the text.