Figure 3.
Optic nerve crush results in loss of βIV spectrin staining at the AIS. A, Immunostaining of control retina for βIV spectrin (green), NeuN (red), and Hoechst (blue). B, Retina corresponding to the crushed optic nerve immunostained for βIV spectrin (green), NeuN (red), and Hoechst (blue). C, Higher magnification of the boxed region shown in A reveals intact AIS (green). D, Higher magnification of the boxed region shown in B demonstrates the loss of βIV spectrin immunostaining. Scale bars: A, B, 50 μm.