Fig. 3.

Effect of topical CEES exposure on protein oxidation in SKH-1 hairless mouse skin. Mice were treated topically with different doses of CEES or acetone alone, and the dorsal skin was collected at specific time points as detailed under Materials and Methods. Skin epidermis samples from both dose- and time-response studies were homogenized in lysis buffer, and lysates were subjected to western blot analysis for protein oxidation as described under Materials and Methods. Twenty µg of protein extracts were incubated with DNPH, subsequently electrophoresed by SDS-PAGE, blotted onto a nitrocellulose membrane, and incubated with polyclonal rabbit anti-DNPH antibody, as detailed under Material and Methods. The gels are representative of two animals in each dose- and time-response study. UC, untreated control; VC, vehicle control.