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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2009 Sep 15;47(11):1640–1651. doi: 10.1016/j.freeradbiomed.2009.09.011

Fig. 6.

Fig. 6

Effect of topical CEES exposure on phosphorylation of PDK1 and Akt in SKH-1 hairless mouse skin. Mice were treated topically with different doses of CEES or acetone alone, and the dorsal skin was collected at specific time points as detailed under Materials and Methods. Western blot analysis was carried out for phospho-PDK1, and phospho- and total Akt in dose-response post 12 h treatment (A) and in time-response study employing 2 mg CEES (B). The membranes were probed with phosphorylated and total antibodies followed by peroxidase-conjugated appropriate secondary antibody as detailed under Materials and Methods. After exposure to X-ray film, the membrane was stripped and reprobed with β-actin as loading control. The results are representative of two animals in each dose- and time-response study. UC, untreated control; VC, vehicle control.