Table 2.
DNA Length (bp) |
Mr (Saturated Complex)a |
Stoichiometry | Sapp bp/protein |
K/M | ω | K•ω |
---|---|---|---|---|---|---|
11 | 5.08 ± 0.24 × 104 |
2.05 ± 0.11a 1.99 ± 0.15b |
5.36 ± 0.31a 5.52 ± 0.38b |
1.69 ± 0.19 × 103 c | 45.9 ± 6.0c | 7.75 ± 1.92 × 104 |
12 | - | 2.91 ± 0.14b | 4.13 ± 0.21b | 4.08 ± 1.12 × 103 c | 48.0 ± 5.5c | 1.96 ± 0.78 × 105 |
14 | - | 2.94 ± 0.10b | 4.75 ± 0.12b | 6.11 ± 0.96 × 103 c | 52.3 ± 5.1c | 3.18 ± 0.82 × 105 |
16 | 9.71 ± 0.17 × 104 |
3.94 ± 0.08a 3.98 ± 0.01b |
4.06 ± 0.08a 4.04 ± 0.02b |
1.25 ± 0.22 × 104 c | 105.2 ± 18.8c | 1.31 ± 0.47 × 106 |
18 | - | 3.82 ± 0.24b | 4.70 ± 0.18b | 4.23 ± 0.72 × 103 c | 85.7 ± 11.6c | 3.59 ± 1.10 × 105 |
21 | - | 5.06 ± 0.20b | 4.15 ± 0.17b | 6.07 ± 1.80 × 103 c | 92.6 ± 25.1c | 5.62 ± 3.21 × 105 |
22 | - | 4.58 ± 0.24b | 4.80 ± 0.24b | 3.80 ± 0.95 × 103 c | 68.3 ± 13.5c | 2.58 ± 1.16 × 105 |
24e | - | 5.93 ± 0.25b | 4.05 ± 0.18b | 2.31 ± 0.36 × 103 c | 83.1 ± 13.2c | 1.92 ± 0.61 × 105 |
24f | - | 6.01 ± 0.20b | 3.99 ± 0.14b | 4.01 ± 1.78 × 103 c | 110.1 ± 23.2c | 4.41 ± 2.89 × 105 |
26 | 1.37 ± 0.41 × 105 |
5.64 ± 0.18a 5.50 ± 0.16b |
4.60 ± 0.18a 4.72 ± 0.14b |
8.18 ± 2.52 × 103 c | 93.5 ± 26.5c | 7.64 ± 4.53 × 105 |
30 | 1.49 ± 0.34 × 105 |
6.08 ± 0.16a 6.43 ± 0.13b |
4.93 ± 0.13a 4.66 ± 0.11b |
9.71 ± 2.03 × 103 c | 64.7 ± 15.3c | 6.28 ± 2.79 × 105 |
41 | - | 9.01 ± 0.24b | 4.55 ± 0.13b | 1.87 ± 0.45 × 103 c | 145.0 ± 37.0c | 2.71 ± 1.35 × 105 |
148 | 5.66 ± 0.14 × 105 |
21.76 ± 0.65a | 6.80 ± 0.21a | 8.23 ± 1.51 × 103 d | 53.5 ± 22.1d | 4.40 ± 2.62 × 105 |
208 | 8.05 ± 0.33 × 105 |
31.04 ± 1.54a | 6.70 ± 0.35a | 7.87 ± 0.82 × 103 d | 63.6 ± 16.6d | 4.95 ± 1.82 × 105 |
2686 | 1.05 ± 0.51 × 107 |
406.3 ± 23.7a | 6.61 ± 0.41a | 8.52 ± 0.96 × 103 d | 22.6 ± 3.7d | 1.92 ± 0.53 × 105 |
Determined by sedimentation equilibrium analysis. The error ranges are 95% confidence limits.
Determined by EMSA-serial dilution analysis. The error ranges are 95% confidence limits.
Determined by scatchard analysis of EMSA data, using binding densities calculated from serial dilution analysis.
Determined by scatchard analysis of sedimentation equilibrium data, using binding densities calculated from Mw of the protein-DNA complex.
dG24•dC24
dA24•dT24