Figure 4.

A, Distribution of prestin-containing OHCs in a chimeric cochlea. A segment of stitched segments lying 2.8 and 4.3 mm from the base is enlarged to reveal the mosaic pattern of OHCs derived from ROSA26 WT and prestin KO embryos. Only OHCs from the ROSA26-positive parent stain with anti-prestin. B, A representative radial section of the organ of Corti in a chimeric mouse is provided in B for illustrative purposes. This 5 μm section was obtained from a mid-cochlear segment (∼3 mm from the helicotrema) at 20× to show increase in Deiters'-cell lengths associated with short, no prestin-containing OHCs. This particular chimera had 39% OHCs staining for prestin. The two shorter than normal OHCs were derived from the prestin KO parent; the longer OHC in row 3, from the ROSA26 WT parent.