Table 1.

Primers for amplification of human NET exons and splice junctions

Name	Sequence (5'–3')	Location	Positiona	Amplicon Size (bp)
Exon 1-F	aaccacctcttttccctttatcca	5' UTR	4662–4685	441
Exon 1-R	tccgtgtgtattccagctcctg	Intron 1	389–410
Exon 2-F	gattgctgcgcgtcgcctttg	Intron 1	79–100	353
Exon 2-R	ccttagatctcaccactggag	Intron 2	411–431
Exon 3-F	catgcgacaggtcactggtg	Intron 2	1–20	466
Exon 3-R	tagtgtttggctcaggtcatac	Intron 3	445–466
Exon 4-F	agagtggccaggtcctgtct	Intron 3	20–39	373
Exon 4-R	cttgcacttccagctccatctt	Intron 4	371–392
Exon 5-F	tggcttcagggccttgcctagag	Intron 4	1–23	341
Exon 5-R	acaagcctggcccaaggcttggt	Intron 5	319–341
Exon 6-F	ctgcccatctctggttcagaccat	Intron 5	11–34	333
Exon 6-R	ggagagttggcttccagaccaga	Intron 6	321–343
Exon 7-F	gtatccatgtggcagcaggagc	Intron 6	6–27	355
Exon 7-R	cacggaagagccatgcagccaa	Intron 7	339–360
Exon 8-F	ctatcatgtgcagctcagaccaatgg	Intron 7	70–95	321
Exon 8-R	gtctgcaatttaaatagggccttctgg	Intron 8	364–390
Exon 9-F	caaggcagcctacatgagtcctgg	Intron 8	15–38	449
Exon 9-R	taacagggctgaatggaatcctcag	Intron 9	439–463
Exon 10-F	cagttcccacgtttgaccaaagagg	Intron 9	646–670	209
Exon 10-R	ggtgcaggattctaggaggactgg	Intron 10	831–854
Exon 11-F	catcttgcctcactgccctgctct	Intron 10	31–54	283
Exon 11-R	atatcctcacccagctccatcctg	Intron 11	290–313
Exon 12-F	ctttgctgtgatgctcacttctcttca	Intron 11	602–628	409
Exon 12-R	ttgaccctagtgtctgtgtccttctg	Intron 12	985–1010
Exon 13-F	gctgcaggatcaaatagcaggtgg	Intron 12	99–122	269
Exon 13-R	gatgtgatgtctagccttgggcagt	Intron 13	343–367
Exon 14-F	cctttctgtccccaccatgtcatc	Intron 13	616–639	126
Exon 14-R	tgcctgtgacctggacattggcat	Intron 14	718–741
Exon 15-F	gagaacatcacatttacgtctactc	Intron 14	3492–3516	233
Exon 15-R	gcttcagtctcacattagcgagg	3' UTR	3702–3724

^aPrimer positions are from NCBI Genbank accession numbers X91117–X91127, or, in the case of primer Exon 1-F, accession number AF061198.

F = forward, R = reverse.