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. 2010 Jan 13;5(1):e8626. doi: 10.1371/journal.pone.0008626

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Sanokawa-Akakura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–D) Eye phenotypes of 15–17 day old flies. (A) wild type flies on 1 mM Cu (B) Aβ flies on normal food. (C) Aβ flies on food supplemented with 1 mM Cu. (D) Aβ flies on food supplemented with 1 mM BCS. (E–F) Quantification of the effects of Cu (E) and BCS (F) feeding on the rough eye phenotype. Phenotypes were evaluated as mild (light gray), moderate (dark gray) or severe (black) and the percent distribution of these phenotypes is shown. The graphs show a shift in the distribution of phenotypes when flies are raised on supplemented food. (G) Dose-dependent increase in levels of copper in flies raised on Cu-supplemented food.