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. 2010 Jan 13;5(1):e8685. doi: 10.1371/journal.pone.0008685

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Ratios of male to female expression (M:F) are presented below each bar. Gene expression, determined by real-time quantitative PCR with SYBR green, is reported relative to the reference gene OnRPS3 on a logarithmic scale. Expression values are presented as averages (with standard error bars) of four biological replicates and three nested technical replicates. Sex-biased expression is supported by nested ANOVA analyses of the normalized CT values, P = 0.03, 0.04, 0.001, 0.06, 0.001 and 0.003, OnOrs1-6 respectively.