Gr-1, mannose receptor (MR), and dectin-1 in wound macrophages. PVA sponges were inserted s.c. in B6D2F1 male mice. Wound cells were obtained 1, 3, or 7 days later. Leukocytes were isolated from blood. Cells were stained for Gr-1, mannose receptor, or dectin-1 (open histograms) or appropriate isotype control antibodies (shaded histograms). Additionally, wound cells were stained for CD68 and blood leukocytes for F4/80. Histograms show expression of antigens in gated macrophages (CD68high cells) or gated monocytes (F4/80+ cells). Results are representative of three experiments.