Figure 2.

Arginase 1 and Ym1, but not iNOS, are present in wound macrophages. Wound cells were obtained 1, 3, or 7 days after PVA sponge insertion. Macrophages were isolated from the wound cell suspensions by negative selection. Cell lysates from unfractionated wound cells or isolated wound macrophages were size-fractionated and immunoblotted with antibodies for iNOS, arginase 1, and Ym1/2. Results are representative of two experiments.