Figure 3.

Wound macrophages express mannose receptor and produce TNF-α. Wound cells were isolated 1 and 7 days after PVA sponge insertion, incubated in complete medium with Brefeldin A for 6 h, and stained with fluorochrome-conjugated antibodies for F4/80, TNF-α, and mannose receptor. The figure shows staining for TNF-α and mannose receptor in gated macrophages (F4/80⁺ cells). Results are representative of two experiments.