TABLE 3.
Plasticity of Wound Macrophages: Cytokine and Chemokine Response to IFN-γ/LPS or IL-4a
| TNF-α (pg) | IL-6 (pg) | IL-10 (pg) | CCL5 (pg) | CCL17 (pg) | |
|---|---|---|---|---|---|
| Untreated | 7.5 ± 9.4 | ND | ND | 21.2 ± 1.1 | 0.5 ± 0.0 |
| IFN-γ and LPS | >1000b | 684 ± 54b | 220 ± 13b | 1264 ± 42b | 0.3 ± 0.1 |
| IL-4 | 14.6 ± 8.5 | 2.1 ± 3.2 | ND | 9.2 ± 4.2 | 14.7 ± 0.4b |
a
Macrophages isolated from Day 7 wound cells were cultured in complete medium for 24 h with or without IL-4 (10 ng/mL) or IFN-γ (10 U/mL) and LPS (0.1 μg/mL). Data are cytokine production/3 × 105 cells, mean ± sd of quadruplicate wells, and are representative of two experiments. IL-12p70 was not detected in culture supernatants.
b
P < 0.05 versus untreated cells, ANOVA with Newman-Keuls.