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. 2009 Oct 2;87(1):69–77. doi: 10.1189/jlb.0509361

Figure 2.

Figure 2.

Decreased migration of MMP-8−/− PMNs through collagen matrices in vivo. To compare PMN infiltration in the tracheal allograft between WT and MMP-8−/− recipients at 2 days and 14 days post-transplant, PMNs were stained with an anti-granulocyte marker (Ly-6C/Ly-6G/Gr-1) and were counted manually inside and outside of the tracheal lumen. Data are expressed as mean ± sem. Analysis of data 2 days post-transplant (n=5) showed significantly less PMNs inside of the tracheal lumen of MMP-8−/− recipients (147.3±33.2) compared with WT recipients (274.3±39.7; P=0.04). There were significantly more PMNs outside of the lumen in WT recipients (1974±467.9) compared with MMP-8−/− recipients (808.9±176.8; P=0.05). Twelve percent of the total cells were present in the lumen in the WT recipients compared with 15% of the total cells in the MMP-8−/− recipient mice. At 14 days post-transplant (n=5), there were significantly less PMNs inside of the tracheal lumen in the MMP-8−/− (129.8±14.9) recipients when compared with the WT recipients (321.8±66.7; P=0.02), and there were significantly more PMNs outside of the lumen in MMP-8−/− recipients compared with the WT recipients (P=0.01). By this time, 38% of the total cells were present in the lumen in the WT recipients compared with only 6% of the total cells in the MMP-8−/− recipient mice.